Amphibians represent the most threatened vertebrate group on the planet, with their population decline being primarily attributable to a number of pressures including, but not limited to, habitat loss, climate change, environmental pollution, the introduction of invasive species and disease infection. In order to enhance the efficacy of amphibian diversity conservation, it is imperative to establish efficient and accurate monitoring techniques to scientifically evaluate the status and dynamics of amphibian populations. Despite the prevalence of conventional morphological and acoustic monitoring techniques, they are encumbered by inherent limitations, including the high financial cost of monitoring, the complexity of species identification, and the challenge of detecting rare species. The utilisation of environmental DNA (eDNA) technology has emerged as a novel approach for the assessment of amphibian diversity, exhibiting notable advantages such as high efficiency, expeditiousness, and non-invasiveness. This paper provides a concise overview of the two primary methods of eDNA biomonitoring, offering a synopsis of the advancements in eDNA technology for the purpose of amphibian “targeted” (invasive species, rare/endangered species) monitoring, biodiversity surveys, species abundance estimation, and pathogenic bacteria monitoring. The focus of this research is the impact of key technical aspects on the accuracy of monitoring, including but not limited to: sample collection and eDNA capture, primer selection and PCR amplification, bioinformatics analysis, and the control of false negatives and false positives. Suggestions for the optimisation of these processes are also put forward. Furthermore, the subsequent research directions and potential applications are discussed in this paper, with the aim of providing a framework for the future monitoring, conservation and management of amphibian diversity.