The actual lakes generally experience repeated wind and wave disturbances, and the mud-water interface process has an important influence on the material circulation of lakes during the cyclic process of suspension and calm settlement. In view of the changes of particulate matter and phosphorus at the mud-water interface in the process of disturbance suspension and calm settlement, repeated experiments of disturbance and settlement were carried out. With the oscillating grating as the disturbance device and 12 h as the period, the intermittent disturbance experiment was carried out for a total of 10 periods, and the change law of turbidity, TP, DTP and particles was paid attention to. The experimental results show that the rise of turbidity, TP and DTP caused by re-suspension will gradually decrease in the process of repeated suspension and settlement, and the greater the disturbance intensity, the more obvious the decline of turbidity, TP and DTP peaks after the disturbance. After repeated disturbance, the particle size of the sediment surface becomes larger, and large floc of about 200~400μm appears. In the process of multiple disturbances, "perturbation-aggregation" occurs between suspended particles and particles, the distance between particles is close to each other, the increase of van der Waals gravity causes flocculation between particles, and the existence of organic matter causes a "bridging effect" between particles, which promotes the formation of larger flocs.