Form 2011 to 2013, benthic macro-invertebrate assemblages were sampled from 31 rivers in Shanghai City. The analyses of the range of index value distribution, judgment ability and Pearson correlation were analyzed on 31 candidate metrics. Four biological metrics including relative percentage of Oligochaeta and Hirudinea, number of tolerant taxa, percentage of Diptera number and percentage of collector taxa richness were selected for establishing Benthic Integrated Biotic Index (B-IBI).The values of metric were calculated by ratio scoring method, and B-IBI was obtained by combining all those 4 indices.According to these established criteria, results showed that among 31 rivers of Shanghai City, 4 rivers were healthy, 4 rivers were sub-healthy, 9 rivers were good-fair, 8 rivers were fair, 2 rivers were poor. The health status was best in rural rivers, followed by suburban rivers, and worst in downtown rivers.