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| 冰封期不同类型湖泊细菌与蓝藻群落组成及共现网络分析 |
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杨文焕1,2, 张明宇1,2, 杨娅婷1,2, 徐龙3, 郭渊3, 马杰1,2, 李卫平1,2
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1.内蒙古科技大学能源与环境学院;2.黄河流域内蒙古段生态保护与综合利用自治区协同创新中心;3.乌兰察布市生态环境局凉城县分局
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| 摘要: |
| 摘 要：季节性冰封使得寒区湖泊微生物群落结构及生物间相互反馈具有特殊性。黄河流域内蒙古段湖泊作为典型的寒区湖泊，其漫长的冰封期为研究细菌与蓝藻在冰封条件下的相互关系提供了天然平台。解析不同类型湖泊生态系统中细菌与蓝藻群落间的相互关系差异，有助于深入理解湖泊冰封条件下水生态系统的功能与动态。为探明湖泊功能对菌—藻关系的影响，本文以大型淡水湖泊乌梁素海、城市湖泊南海湖、咸水湖泊岱海冰封期冰下水体为研究对象，利用宏基因测序分子生物学技术、冗余分析、Mantel检验分析及Network网络分析模型等方法，对冰封期间细菌和蓝藻群落结构及相互关系进行研究。结果表明：3个湖泊冰下水体中优势菌门组成一致，属水平上的群落组成具有较为明显差异；湖泊间菌藻群落组成差异与环境因子的协同作用高度相关，总磷、氨氮、总氮是影响冰封期菌藻群落结构的主要影响环境因子；细菌—蓝藻的微生物网络复杂性呈现南海湖＞岱海＞乌梁素海。岱海网络中菌藻关系多以负相关为主，负权重边占比达到31.70%，且模块化程度较高。本文旨在对湖泊冰下水体中的细菌、蓝藻群落结构及共现网络进行深入分析，以更好地理解不同水生态系统下微生物错综复杂的关系，为相关领域的生态保护和研究提供重要的理论依据。 |
| 关键词: 冰封期 宏基因 群落结构 冗余分析 菌藻关系 |
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| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目）(42167018)资助 |
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| Co-occurrence network analysis and community composition of bacteria and cyanobacteria in various types of lakes during the frozen period |
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yang wen huan1,2, zhang ming yu1,2, yang ya ting1,2, Xu long3, Guo yuan3, ma jie1,2, li wei ping1,2
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1.College of Energy and Environment，Inner Mongolia University of Science and Technology;2.Autonomous Region Level Ecological Protection and Comprehensive Utilization Cooperative Innovation Center for the Inner Mongolia Section of the Yellow River;3.Liangcheng County Branch of Ecological Environment Bureau of Ulanqab City
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| Abstract: |
| Due to seasonal freezing, the biological community succession in lakes and the reciprocal feedback between species are disparate in cold climates. As typical lakes in the Inner Mongolia section of the Yellow River Basin, the long freezing period provides a natural platform for the study of bacterial and cyanobacterial interactions under freezing conditions. Clarifying the differences in the interrelationships between bacterial and cyanobacterial communities in different types of lake ecosystems will help us to gain a deeper understanding of the functioning and dynamics of the aquatic ecosystems of the lakes under freezing conditions. To find out whether the different functions of lakes produce differences in the bacteria-algae relationship, this paper used the subglacial water of Lake Ulansuhai, Nanhai Lake, and Daihai Lake as the research objects. Lake Ulansuhai is a rare large grassland lake in the global desert and semi-desert areas, plays an important role in maintaining the ecological balance of the Yellow River Basin, and is a representative of the freshwater lakes in the cold and arid areas. Daihai Lake is the third largest inland lake in Inner Mongolia, and Hulun Lake, Lake Ulansuhai is jointly known as "a lake and two seas" is an important ecological barrier in the northern border of China, 2015 ~ 2020 Daihai Lake average salinity of the water body reached 12.82 ‰, with the same latitude compared to the salinity of lakes in the middle to high level, is a typical inland saltwater lake. Nanhai Lake is located in Baotou City, Inner Mongolia, as a small urban shallow lakes affected by both natural and man-made factors, not only has the characteristics of lakes in cold and dry areas is also a typical representative of small urban lakes. In this paper, the community structure and relationships between freezing period bacteria and cyanobacteria were investigated using metagenomic sequencing molecular biology technology, redundancy analyses, Mantel test analyses, and network analysis models. The findings demonstrated that the four major phyla in the three lakes were Proteobacteria, Bacteroidetes, Actinobacteria, and Cyanobacteria during the freezing period. Significant differences in bacterial and cyanobacterial community composition at the genus level in the Lake Ulansuhai, Dahai Lake, and Nanhai Lake. There exists a strong correlation between the variations in algal and bacterial community composition among the three lakes and the synergistic effect of environmental conditions. The primary cause of the variations in the algal and bacterial community structures is water ecological conditions. It is noteworthy that salinity has a significant impact on the composition of the microbial community in Daihai Lake. The ammonia nitrogen(NH4+-N) concentration has a big impact on Nanhai Lake, and environmental conditions have a big impact on the percentage of cyanobacteria abundance in the three lakes. The analysis of the bacteria-cyanobacteria network revealed that Nanhai Lake was more complicated than Daihai Lake and Lake Ulansuhai. More bacteria and algae had competitive antagonistic relationships in Daihai Lake, which is known for its high salinity; also, the bacteria-cyanobacteria network in Daihai Lake and Lake Ulansuhai shown greater stability. In order to better understand the abiotic factors affecting the co-evolution of bacteria and cyanobacteria in various aquatic ecosystems, the aim of this paper to sort out the abiotic factors of dominant bacteria and cyanobacteria in the aforementioned three different types of lakes. It also analyzes the significant synergistic relationship between bacteria and cyanobacteria in the three lakes. Finally, it serves as a reference for anticipating and managing water quality issues that may arise during the lake"s freezing period. |
| Key words: Frozen period Metagenome Community structure Redundancy analysis Bacteria-algae interactions |
