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| 青藏高原东缘溪流型小微湿地沉积物古菌群落的海拔分布格局及驱动因素 |
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郭云1, 仲波2, 李娜英3, 李贤祥4, 张慧2, 杨早2, 蒋海波2, 王晓锋4, 杨刚5, 何奕忻6
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1.西南科技大学生命科学与工程学院，中国科学院成都生物研究所;2.中国科学院成都生物研究所;3.中国科学院成都生物研究所，四川大学生命科学学院;4.重庆师范大学地理与旅游学院;5.西南科技大学生命科学与工程学院;6.中科院成都生物研究所
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| 摘要: |
| 小微湿地广泛分布于河流源头区域，对维持生态系统稳定性以及提供生态系统服务发挥着重要作用，微生物在其中扮演着重要角色，但当前对小微湿地沉积物中微生物群落尤其是古菌群落的海拔分布格局尚不清楚。因此，基于16S rRNA基因测序技术，本研究调查了岷江上游5个海拔梯度（1000、1600、2000、2700 和3200 m）的溪流型小微湿地的水文特征、水体理化性质、沉积物古菌群落的组成和多样性等指标。结果表明，岷江上游溪流型小微湿地沉积物中古菌群落组成在海拔梯度上存在显著差异，群落α多样性随海拔升高而显著增加，β多样性在海拔梯度上差异显著。距离衰减分析表明，古菌群落的时空分布与海拔梯度显著相关，进一步的冗余分析证实了浊度、pH及碳氮（溶解性有机碳、溶解性总氮、总氮等）是驱动岷江上游溪流型小微湿地沉积物中古菌群落分布格局的重要环境因素。综上所述，本研究厘清了岷江上游溪流型小微湿地沉积物古菌群落沿海拔梯度的分布格局，揭示了溪流型小微湿地中水文和水体理化性质共同调控沉积物古菌群落的海拔分布格局，为理解青藏高原自然小微湿地古菌群落的海拔分布的生物地理分布提供了科学依据。 |
| 关键词: 古菌 群落组成 多样性 水体理化 水文 |
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| 基金项目:中国博士后科学基金,国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| Elevational distribution pattern and driving factors of archaeal communities in the sediments of small streams along the eastern Tibetan Plateau* |
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guo yun,zhong bo,li na ying,li xian xiang,zhang hui,yang zao,jiang hai bo,wang xiao feng,yang gang,He Yixin
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1.School of Life Science and Engineering, Southwest University of Science and Technology，Chengdu Institute of Biology, Chinese Academy of Sciences;2.Chengdu Institute of Biology, Chinese Academy of Sciences;3.Chengdu Institute of Biology, Chinese Academy of Sciences，College of Life Sciences, Sichuan University;4.College of Geography and Tourism, Chongqing Normal University;5.School of Life Science and Engineering, Southwest University of Science and Technology
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| Abstract: |
| Small and micro wetlands including small streams are widely distributed in the source areas of rivers, which play an important role in maintaining the ecosystem stability and providing ecosystem services. However, the distribution of microbial communities, especially the archaeal communities, in the stream sediments along different elevations is still unclear. To supplement the theoretical framework within this field, we conducted a study in five stream type small micro wetlands of the upper Minjiang River along different altitudinal gradient (1000, 1600, 2000, 2700 and 3200 m) by using 16S rRNA illumina sequencing technology and monitoring the physicochemical properties of the water body as well as hydrological parameters. The alpha diversity of the archaeal community increased significantly with elevation, and the beta diversity varied significantly across the elevation gradient. Habitat difference was the key factor driving the altitudinal distribution pattern of archaeal communities in the stream type small micro wetlands sediments of the upper Minjiang River. Total dissolved nitrogen, total nitrogen, total dissolved phosphorus and pH were the key physicochemical factors affecting the community structure, while turbidity and water depth were the key hydrological factors affecting the community structure. In conclusion, this study showed that hydrological factors and water body physicochemical properties jointly regulate the altitudinal distribution pattern of stream type small micro wetlands archaeal communities. This study provides a scientific basis for understanding the biogeographic distribution of the altitudinal distribution of natural small micro wetland archaeal communities in the Tibetan Plateau. |
| Key words: Archaea community composition diversity physical and chemical properties of water body hydrology |
