| 引用本文: | 孙继贤,廖家遗.罗氏沼虾胸神经节中促进卵母细胞发育的激素的初步分离.湖泊科学,2003,15(1):64-69. DOI:10.18307/2003.0109 |
| SUN Jixian,LIAO Jiayi.Preliminary Isolation of A Hormone Stimulating Oocyte Development from Thoracic Ganglion of the Prawn, Macrobrachium rosenbergii. J. Lake Sci.2003,15(1):64-69. DOI:10.18307/2003.0109 |
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| 摘要: |
| 罗氏沼虾成虾的胸神经节匀浆经Sephadex-G50柱层析所得第2个峰再经HPLC分离得到9个组分.第1个组分和罗氏沼虾期卵巢小块一起体外培养36h,可使卵母细胞的直径较对照组非常显著地增大(P<0.01),这种增大卵母细胞直径的能力随该组分中蛋白质浓度的增加而增加.在该组分中的激素分子量在1.3kDa左右.结果显示罗氏沼虾胸神经节能分泌促进卵母细胞发育的激素. |
| 关键词: 罗氏沼虾 胸神经节 卵母细胞发育 激素分离 |
| DOI：10.18307/2003.0109 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目30070105. |
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| Preliminary Isolation of A Hormone Stimulating Oocyte Development from Thoracic Ganglion of the Prawn, Macrobrachium rosenbergii |
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SUN Jixian, LIAO Jiayi
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School of Life Sciences, Zhongshan University, Guangzhou 510275, P. R. China
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| Abstract: |
| The extracts of thoracic ganglion from adult Macrobrachium rosenbergii were chromatographed on a Sephadex G-50 column, the resulting second peak was further isolated by HPLC, and 9 fractions were obtained. Among the fractions only the first fraction significantly increased the oocyte diameter in vitro of the stage Ⅲ ovary in M. rosenbergii. The increasing effects were depended on the protein concentration of the fraction for culture. The molecular weight of the substance in the fraction 1 was about 1.3kDa.The results show that the thoracic ganglion of M. rosenbergii can secrete peptide hormone which stimulates the oocyte development of the prawn. |
| Key words: Macrobrachium rosenbergii thoracic ganglion oocyte development Hormone isolation |
