| 引用本文: | 谭啸,孔繁翔,曹焕生,史小丽,于洋,张民.利用流式细胞仪研究温度对两种藻竞争的影响.湖泊科学,2006,18(4):419-428. DOI:10.18307/2006.0415 |
| TAN Xiao,KONG Fanxiang,CAO Huansheng,SHI Xiaoli,YU Yang,ZHANG Min.Influences of temperatures on the competition between two species of algae assayed by flow cytometry. J. Lake Sci.2006,18(4):419-428. DOI:10.18307/2006.0415 |
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| 利用流式细胞仪研究温度对两种藻竞争的影响 |
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谭啸1,2, 孔繁翔1, 曹焕生1,3, 史小丽1, 于洋1, 张民1,3
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1.中国科学院南京地理与湖泊研究所, 南京 210008;2.南京师范大学生命科学学院, 南京 210097;3.中国科学院研究生院, 北京 100039
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| 摘要: |
| 为研究温度对微囊藻优势形成的影响,本试验利用流式细胞仪研究了不同温度时铜绿微囊藻(Microcystis aerugi-nosa与蛋白核小球藻(Chlorella pyrenoidosa)间的竞争关系,在单独培养和混合培养条件下分别对两种藻细胞密度比值和细胞内叶绿素a(Chla)荧光强度进行了测定．结果表明在本试验条件下,随着温度的升高(18℃,25℃和32℃),混合培养组中微囊藻优势逐渐明显,小球藻Chla荧光强度随着温度的升高而升高,微囊藻Chla荧光强度随着温度的升高却有所降低．说明在光照适宜、高营养盐的水体中,较高的温度对铜绿微囊藻成为优势种具有明显的促进作用,进而影响着微囊藻水华的形成． |
| 关键词: 流式细胞仪 温度 铜绿微囊藻 蛋白核小球藻 竞争 |
| DOI：10.18307/2006.0415 |
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| 基金项目:国家重点基础研究发展计划(2002CB412305);国家自然科学基金(40471045);中国科学院南京地理与湖泊研究所所长基金联合资助 |
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| Influences of temperatures on the competition between two species of algae assayed by flow cytometry |
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TAN Xiao1,2, KONG Fanxiang1, CAO Huansheng1,3, SHI Xiaoli1, YU Yang1, ZHANG Min1,3
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1.Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P. R. China;2.College of Life Science, Nanjing Normal University, Nanjing 210097, P. R. China;3.Graduate School, Chinese Academy of Sciences, Beijing 100039, P. R. China
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| Abstract: |
| To detect the influences of different temperatures on Microcystis aeruginosa dominance, this experiment researched competition between Microcystis aeruginosa and Chlorella pyrenoidosa under different temperatures by flow cytometry, cell density ratio and chlorophyll-a fluorescence intensity in two species of algae cell were measured. Results displayed under this experimental condition Microcystis aeruginosa dominance became more evident together with temperature rised( 18℃,25℃ and 32℃ ). Higher temperature enhanced chlorophyll-a fluorescence intensity in Chlorella pyrenoidosa cell but reduced chlorophyll-a fluorescence intensity in Microcystis aeruginosa cell. This experiment indicated under suitable light and high nutrition salt condition, higher temperature promoted Microcystis aeruginosa dominance obviously and influenced water bloom forming. |
| Key words: Flow cytometry temperature Microcystis aeruginosa Chlorella pyrenoidosa competition |
