| 引用本文: | 钟立强,张成锋,周凯,李冰,王建新,朱健.四个鲤鱼种群ITS-1序列的遗传变异分析.湖泊科学,2011,23(2):271-276. DOI:10.18307/2011.0217 |
| ZHONG Liqiang,ZHANG Chengfeng,ZHOU Kai,LI Bing,WANG Jianxin,ZHU Jian.Sequence variation of Ribosomal DNA Internal Transcribed Spacer 1 of four common carp populations. J. Lake Sci.2011,23(2):271-276. DOI:10.18307/2011.0217 |
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| 四个鲤鱼种群ITS-1序列的遗传变异分析 |
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钟立强1,2,3, 张成锋1, 周凯1,4, 李冰1, 王建新1, 朱健1,3,4
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1.中国水产科学研究院淡水渔业研究中心, 无锡 214081;2.江苏省淡水水产研究所, 南京 210017;3.上海海洋大学水产与生命科学学院, 上海 201306;4.南京农业大学无锡渔业学院, 无锡 214081
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| 摘要: |
| 我国四个鲤鱼种群的核糖体DNA内转录间隔区ITS-1进行了PCR扩增、测序.序列分析显示, 370bp的ITS—l序列中GC含量明显高于AT含量, 共检测到34个变异位点, 96个样本得到14个单倍型, 平均单倍型多样性为0.637±0.055, 核苷酸多样性为0.00857±0.00200, 遗传多样性表现较低.黑龙江野鲤种群与建鲤种群问遗传距离最远, 为0.10129, 黑龙江野鲤种群与黄河鲤种群间遗传距离最近, 为0.02305.分子方差分析(AMOVA)表明, 群体间遗传分化系数Fst为0.05373, 几乎所有变异都来自群体内, 群体间遗传分化极小.ITS-1序列构建系统进化树显示, 四个种群分为南北2支, 黑龙江野鲤和黄河鲤种群聚为北方支, 建鲤和荷包红鲤种群聚为南方一支。 |
| 关键词: 鲤鱼 内转录间隔区1(ITS-1) 种质资源 遗传变异 |
| DOI：10.18307/2011.0217 |
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| 基金项目:中央级公益性科研院所基本科研业务费专项基金项目(2009JBFBl4)、现代农业产业技术体系建设专项资金项目(NYCYTX-49)和农业部种质资源保护项目联合资助 |
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| Sequence variation of Ribosomal DNA Internal Transcribed Spacer 1 of four common carp populations |
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ZHONG Liqiang1,2,3, ZHANG Chengfeng1, ZHOU Kai1,4, LI Bing1, WANG Jianxin1, ZHU Jian1,3,4
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1.Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, P.R.China;2.Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, P.R.China;3.Fisheries and Life Science College, Shanghai Ocean University, Shanghai 201306, P.R.China;4.Fisheries College, Nanjing Agricultural University, Wuxi 214081, P.R.China
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| Abstract: |
| The internal transcribed spacer 1 (ITS-1) of four common carp populations were amplified and sequenced.The result showed that the average contents of GC were obviously higher than that of AT in the aligned sequences of 370bp.Total 34 variable sites were detected, and 14 haplotypes were recovered.The haplotype diversity and nucleotide diversity were 0.637±0.055 and 0.00857±0.00200, respectively.The genetic distance between Heilongjiang carp and Jian carp displayed the highest, up to 0.10129, while that between Heilongjiang carp and Yellow River carp peculations was the lowest to 0.02305.The fixation indices (Fst) of analysis of molecular variance (AMOVA) among populations was 0.05373, which showed no significant population structure.Tlie phylogenetic tree of ITS-1 built with MEGA 4.1 showed that four common carp populations were clustered into two major clades based on genetic distance.Populations of Heilongjiang carp and Yellow River carp were clustered in northern clade, while Jian carp and Hebao red carp were clustered in southern clade. |
| Key words: Common carp interna]transcribed spacer 1 genetic resources genetic diversity |
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