| 引用本文: | 田秀丽,施国新,徐君,贾荣,乔绪强,徐小颖,杨海燕,王娟.Cr6+胁迫对水花生(Alternanthera philoxeroides)愈伤组织多胺代谢的影响.湖泊科学,2011,23(6):955-960. DOI:10.18307/2011.0619 |
| TIAN Xiuli,SHI Guoxin,XU Jun,JIA Rong,QIAO Xuqiang,XU Xiaoying,YANG Haiyan,WANG Juan.Effect of chrome stress on polyamine metabolism of callus of Alternanthera philoxeroides. J. Lake Sci.2011,23(6):955-960. DOI:10.18307/2011.0619 |
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| Cr6+胁迫对水花生(Alternanthera philoxeroides)愈伤组织多胺代谢的影响 |
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田秀丽, 施国新, 徐君, 贾荣, 乔绪强, 徐小颖, 杨海燕, 王娟
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南京师范大学生命科学学院, 江苏省生物多样性与生物技术重点实验室, 南京 210046
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| 摘要: |
| 利用植物组织培养技术培养水花生愈伤组织并以此为实验材料, 研究了不同Cr6+浓度(0、0.1、0.3、0.6、0.9mmol/L)对水花生愈伤组织中腐胺(putrescine, Put)、亚精胺(spermidine, Spd)、精胺(spermine, Spm)、脯氨酸(Pro)含量及精氨酸脱羧酶(ADC)、鸟氨酸脱羧酶(ODC)、多胺氧化酶(PAO)和二胺氧化酶(DAO)活性的影响.结果表明, 随着Cr6+处理浓度的增大:(1)游离态腐胺(f-Put)、亚精胺(f-Spd)和精胺(f-Spm)的含量先升后降, 且均在0.6mmol/L时达到峰值;(2)结合态腐胺(结合态Put)含量在0.1mmol/L时达到最大值, 之后下降;而结合态Spd和结合态Spm含量则逐渐上升;(3)束缚态Put、束缚态Spd和束缚态Spm含量先升后降, 在0.6mmol/L时束缚态Put与Spd达到最大值;而束缚态Spm则是在0.1mmol/L时达到最大值, 之后逐渐下降;(4)ADC酶和ODC酶活性逐渐上升;而PAO、DAO酶活性则表现为先升后降;(5)Pro含量呈先升后降趋势.可见, 重金属Cr6+胁迫下, 破坏了水花生愈伤组织内多胺代谢平衡, 造成多胺(polyamines, PAs)大量积累, 较高的内源PAs在提高愈伤组织抵抗Cr6+胁迫能力的过程中发挥重要作用。 |
| 关键词: 愈伤组织 水花生 Cr6+胁迫 多胺 代谢 |
| DOI：10.18307/2011.0619 |
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| 基金项目:国家自然科学基金项目(30870139)资助 |
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| Effect of chrome stress on polyamine metabolism of callus of Alternanthera philoxeroides |
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TIAN Xiuli, SHI Guoxin, XU Jun, JIA Rong, QIAO Xuqiang, XU Xiaoying, YANG Haiyan, WANG Juan
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Jiangsu Key Laboratory of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, P.R.China
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| Abstract: |
| Callus of Alternanthera philoxeroides, as experimental material, was cultured by tissue culture technique.In this study, the calluses were subjected to different concentrations of Cr6+ (0, 0.1, 0.3, 0.6 and 0.9 mmol/L) and the impacts on contents of putrescine (Put), spermidine (Spd), spermine (Spm)and proline(Pro), activities of arginine decarboxylase (ADC), orni-thine decarboxylase(ODC), polyamine oxidase(PAO) and Diamine Oxidase(DAO) were investigated.Results indicated that, with the increasing of Cr6+ concentration, (1) the contents of three free PAs increased at first and then decreased, and reached their peak values at 0.6 mmol/L Cr6+ treatments;(2) the content of PS-conjugated Put reached its peak value at 0.1 mmol/L Cr6+ treatment and then decreased slowly, while the contents of PS-conjugated spermidine (Spd) and spermine (Spm) increased significantly;(3) the peak values of PIS-conjugated Put and Spd were present at 0.6 mmol/L Cr6+ treatment;meantime the peak value of PIS-conjugated Spm was present at 0.1 mmol/L Cr6+ treatment;(4)the activities of ADC and ODC increased gradually, while PAO and DAO activities decreased sharply after a transient increase;(5) the level of Pro increased at 0.1-0.3 mmol/L Cr6+ treatments and decreased at 0.6-0.9 mmol/L Cr6+ treatments.These results suggested that Cr6+ stress disturbed metabolic balance of polyamines and increased the level of PAs.Obviously, the changes of PAs levels might be essential to Cr6+ adaption of cells. |
| Key words: Callus Alternanthera philoxeroides Cr6+ stress polyamines metabolism |
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