| 引用本文: | 黎丽雯,邹立扣,周杨,胡苏,庞玉娟.成都府河水体细菌动态变化及耐药性.湖泊科学,2012,24(1):96-103. DOI:10.18307/2012.0113 |
| LI Liwen,ZOU Likou,ZHOU Yang,HU Su,PANG Yujuan.Dynamics of bacterial diversity and antibiotic resistance of Escherichia coli in Fu River, Chengdu. J. Lake Sci.2012,24(1):96-103. DOI:10.18307/2012.0113 |
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| 成都府河水体细菌动态变化及耐药性 |
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黎丽雯1,2,3, 邹立扣1, 周杨1,2, 胡苏1,2, 庞玉娟1
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1.四川农业大学都江堰校区微生物学实验室, 都江堰 611830;2.四川农业大学城乡建设学院, 都江堰 611830;3.中国科学院南京地理与湖泊研究所, 南京 210008
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| 摘要: |
| 将府河从都江堰宝瓶口段至望江公园段分设9个监测点,在秋冬、春夏两季分别取水,首次建立由细菌总数、总大肠菌群数及粪大肠菌群数三个微生物学指标组成的体系,对府河两季水质进行对比监测分析,以综合评价水质、分析季节影响因素并反映出污染物来源.以都江堰宝瓶口大肠杆菌耐药率作为背景值,对整个河段的9个采样点分离纯化鉴定得到102株大肠杆菌,用K-B法进行对比药敏试验.结果表明,府河从都江堰段起,穿过成都市区后,至望江公园段细菌总数、大肠菌群数及粪大肠菌群数虽有波动,但细菌总数总体呈增加趋势,春夏季府河营养富集,水质有所降低.府河中大肠杆菌耐药性较小,β-内酰胺类耐药率在0.96%~33.33%之间,氨基糖苷类1.39%~7.84%,氟喹诺酮类11.43%~11.76%,磺胺类复方新诺明为52.78%,其中β-内酰胺类耐药性、氟喹诺酮类耐药性与源头水质有关,其余与医疗机构排放的废水及生活污水有关,此研究为控制成都府河水体中的污染防控提供了理论依据. |
| 关键词: 府河 细菌动态 水质评价 耐药性 大肠杆菌 |
| DOI：10.18307/2012.0113 |
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| Dynamics of bacterial diversity and antibiotic resistance of Escherichia coli in Fu River, Chengdu |
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LI Liwen1,2,3, ZOU Likou1, ZHOU Yang1,2, HU Su1,2, PANG Yujuan1
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1.Lab of Microbiology, Sichuan Agricultural University, Dujiangyan 611830, P. R. China;2.Urban and Rural Development College, Sichuan Agricultural University, Dujiangyan 611830, P. R. China;3.Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P. R. China
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| Abstract: |
| To analyze the water quality of the Fu River, nine sampling sites were chosen from Baopingkou site in Dujiangyan to Wangjiang Park site in Fu River. Three microbiological indexes, including total number of bacteria, total coliform and the number of fecal coliform, have been selected to evaluate the water quality, analyze seasonal factors and find out the contamination sources in two different seasons. Besides, taking the antibiotic resistance rate of Escherichia coli at Baopingkou as background, 102 E. coli identified by morphology and 16S rDNA have been selected for following antimicrobial susceptibility testing, and the susceptibility has been performed according to the standard disk diffusion K-B method. The results show that though the total number of bacteria, total coliform and fecal coliform varied from Baopingkou, Dujiangyan to Wangjiang Park, the total number of bacteria shows an increasing trend as the water flows. The nutrients enrich in spring and summer, and water quality decrease. The susceptibility of 13 antimicrobial agents tested against E. coli isolated shows that 0.96% 33.33% of the isolates are resistant to β-lactam, 1.39% 7.84% to aminoglycoside, 11.43% 11.76% to fluoroquinolone, and 52.78% to trimethoprim-sulfamethoxazole. The resistance to β-lactam and fluoroquinolone is related with the source of water, however, other resistances are in connection with polluted water discharged from hospital and residential areas. Generally, this study has provided theoretical foundation for the control of pollution in Fu River. |
| Key words: Fu River bacterial diversity water quality analysis antibiotic resistance Escherichia coli |
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