| 引用本文: | 蒋丽娟,尹颖,杨柳燕,尹大强,王晓蓉,郭红岩.菲对斜生栅藻(Scenedesmus obliquus)自由基的诱导及胁迫效应.湖泊科学,2013,25(6):943-949. DOI:10.18307/2013.0619 |
| JIANG Lijuan,YIN Ying,YANG Liuyan,YIN Daqiang,WANG Xiaorong,GUO Hongyan.Free radical generation and stress effect on Scenedesmus obliquus exposed to phenanthrene. J. Lake Sci.2013,25(6):943-949. DOI:10.18307/2013.0619 |
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| 菲对斜生栅藻(Scenedesmus obliquus)自由基的诱导及胁迫效应 |
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蒋丽娟1, 尹颖1, 杨柳燕1, 尹大强2, 王晓蓉1, 郭红岩1
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1.南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210046;2.同济大学环境科学与工程学院, 长江水环境教育部重点实验室, 上海 200092
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| 摘要: |
| 通过静态模拟实验,研究不同浓度(0.005、0.01、0.025、0.05和0.1 mg/L)菲暴露96 h后,对斜生栅藻的生长、自由基含量、抗氧化系统以及脂质过氧化产物(MDA)的影响.结果表明,0.01 mg/L菲开始对藻细胞生长产生显著抑制作用,在此浓度下电子自旋共振检测到的自由基信号(g=2.0033,幅宽10.15 mT)显著增强;在浓度设置范围内,超氧化物酶歧化酶、过氧化物酶、谷胱甘肽-S-转移酶活性均被显著诱导,显示出抗氧化系统的应激机制.谷胱甘肽和MDA含量均在0.025 mg/L时被显著诱导.这说明斜生栅藻对菲暴露比较敏感,0.01 mg/L即已显示胁迫效应,自由基的诱导是斜生栅藻产生损伤的重要原因,藻细胞的生长量是比较敏感的损伤指标. |
| 关键词: 菲 斜生栅藻 电子自旋共振 自由基 氧化胁迫 |
| DOI：10.18307/2013.0619 |
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| 基金项目:黄河流域公益性项目(201001010);国家自然科学基金项目(20907020)联合资助 |
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| Free radical generation and stress effect on Scenedesmus obliquus exposed to phenanthrene |
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JIANG Lijuan1, YIN Ying1, YANG Liuyan1, YIN Daqiang2, WANG Xiaorong1, GUO Hongyan1
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1.State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210046, P. R. China;2.Laboratory of Yangtze River Water Environment, Ministry of Education, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, P. R. China
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| Abstract: |
| In this study,indoors static simulation test was used to investigate the effect of different exposure levels (0.005,0.01, 0.025,0.05 and 0.1 mg/L) of phenanthrene on the growth,free radical,antioxidant defenses and malondialdehyde in Scenedesmus obliquus.Results showed that after 96-h exposure at 0.01 mg/L,the growth of algal cells were significantly inhibited and the signal of free radical (g=2.0033,linewidth is 10.15 mT) increased.The superoxide dismutase,catalase,glutathione-s-transferase activities increased significantly after phenanthrene exposure,demonstrating inducibility mechanisms under stress.The glutathione and malondialdehyde content were significantly elevated after exposure to 0.025 mg/L phenanthrene.In conclusion,S.obliquus is sensitive to phenanthrene,since phenanthrene exposure at low level (even 0.01 mg/L) can induce stress effect.The production of free radical may be an important mechanism involved in the toxicities of phenanthrene on S.obliquus,among which the algae growth is more sensitive to damage index. |
| Key words: Phenanthrene Scenedesmus obliquus electron paramagnetic resonance (EPR) free radical antioxidant stress |
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