| 引用本文: | 于婷,戴景峻,雷腊梅,彭亮.温度、光照强度及硝酸盐对拟柱孢藻(Cylindrospermopsis raciborskii N8)生长的影响.湖泊科学,2014,26(3):441-446. DOI:10.18307/2014.0315 |
| YU Ting,DAI Jingjun,LEI Lamei,PENG Liang.Effects of temperature, irradiance and nitrate on the growth of Cylindrospermopsis raciborskii N8. J. Lake Sci.2014,26(3):441-446. DOI:10.18307/2014.0315 |
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| 摘要: |
| 以从南亚热带水库中分离的拟柱孢藻(Cylindrospermopsis raciborskii N8)为材料,研究了不同温度(12、16、20、24、28、32℃)、光照强度(6.6、12.4、21.5、30.7、62.9、106.4μmol/(m2·s))和硝态氮浓度(0.5、1、2、4、8、16、32、64、128 mg/L)下拟柱孢藻的生长特性.结果表明:在实验设置的温度范围(16~32℃)内拟柱孢藻能够正常生长;最适温度范围为24~28℃,在28℃条件下,具有最大比生长速率,为0.189 d-1;当温度为12℃时,拟柱孢藻叶绿素a浓度一直降低,显著低于其他温度组(16~32℃).在6.6~106.4μmol/(m2·s)光照强度范围内,拟柱孢藻均呈指数增长趋势,最适光照强度为30.7μmol/(m2·s),其比生长速率达到最大值,为0.156 d-1;高光照强度(62.9~106.4μmol/(m2·s))下拟柱孢藻的比生长速率显著大于低光照强度(6.6~12.4μmol/(m2·s))处理组.拟柱孢藻开始指数增长的最低硝态氮浓度为4 mg/L;硝态氮浓度为8 mg/L时,拟柱孢藻达到最大比生长速率(0.155 d-1);当硝态氮浓度高于16 mg/L时比生长速率增加不显著.高硝态氮浓度组(16~128 mg/L)拟柱孢藻的叶绿素a浓度和比生长速率显著高于低硝态氮浓度组(0.5~2 mg/L).研究结果说明拟柱孢藻对温度、光照和氮源均有较宽的生态位,有利于在较大空间尺度上进行扩散. |
| 关键词: 拟柱孢藻 生长 比生长速率 温度 光照强度 硝酸盐 |
| DOI：10.18307/2014.0315 |
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| 基金项目:国家自然科学基金项目(31070416);暨南大学科研培育与创新基金项目(11612322);国家级大学生创新创业训练计划项目(1210559020)联合资助 |
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| Effects of temperature, irradiance and nitrate on the growth of Cylindrospermopsis raciborskii N8 |
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YU Ting, DAI Jingjun, LEI Lamei, PENG Liang
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Department of Ecology and Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, P. R. China
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| Abstract: |
| Cylindrospermopsis raciborskii N8 strain was isolated from a sub-tropical reservoir in southern China and cultured with BG-11 culture medium. The effects of temperature, irradiance and nitrate on the growth of this strain were evaluated by a designed experiment with gradients of temperature (12, 16, 20, 24, 28, 32℃), irradiance(6.6, 12.4, 21.5, 30.7, 62.9, 106.4 μmol/(m2·s)) and nitrate (0.5, 1, 2, 4, 8, 16, 32, 64, 128 mg/L). C. raciborskii N8 had normal growth at temperature between 16℃ and 32℃, and the maximum specific growth rate (0.189 d-1) were observed at temperature between 24℃ and 28℃; C. raciborskii N8 could not survive at 12℃, and chlorophyll-a concentration was significantly lower than other temperature treatments (16-32℃). C. raciborskii N8 had a wide adaptation of irradiance ranging from 6.6 μmol/(m2·s) to 106.4 μmol/(m2·s). An exponential growth was found from 6.6 to 106.4 μmol/(m2·s). The optimal irradiance was 30.7 μmol/(m2·s) and the maximum specific growth rate was 0.156 d-1. C. raciborskii N8 grew faster significantly in high irradiance conditions (62.9-106.4 μmol/(m2·s)) than that in low irradiance conditions (6.6-12.4 μmol/(m2·s)). The species began an exponential growth under nitrate of 4 mg/L, reached the highest specific growth rate (0.155 d-1) at the nitrate concentration of 8 mg/L. The specifical growth rates became stable when the nitrate concentration exceeded 16 mg/L. C. raciborskii N8 grew faster significantly in the high nitrate conditions (16-128 mg/L) than that in the low nitrate conditions (0.5-2 mg/L). The present experiments demonstrated that C. raciborskii N8 can adapt for wide temperatures, irradiance and nitrates, and has a potential to colonize and distribute in divers water body over a large scale. |
| Key words: Cylindrospermopsis raciborskii N8 growth specific growth rate temperature irradiance nitrate |
