| 引用本文: | 唐诗琴,王庆,刘璐,杨宇峰.基于环境DNA宏条形码技术的水体无脊椎动物多样性研究:以广州海珠湖为例.湖泊科学,2023,35(4):1443-1456. DOI:10.18307/2023.0437 |
| Tang Shiqin,Wang Qing,Liu Lu,Yang Yufeng.Biodiversity of aquatic invertebrates based on environmental DNA metabarcoding technology: A case study of Lake Haizhu in Guangzhou. J. Lake Sci.2023,35(4):1443-1456. DOI:10.18307/2023.0437 |
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| 摘要: |
| 于2021年1月,利用环境DNA宏条形码(eDNA)技术,对广州海珠国家湿地公园海珠湖的水体和沉积物进行无脊椎动物多样性和群落结构调查,并比较了eDNA和传统形态学鉴定对浮游动物的检出能力。结果表明:eDNA检出海珠湖无脊椎动物9门16纲34目71科93属137个可操作分类单元(OTUs);其中,水采样品中共检出9门50属68个OTUs,网采样品中共检出6门27属35个OTUs,沉积物休眠卵样品中共检出9门70属103个OTUs。水采和网采样品中,主要的无脊椎动物均为轮虫动物门和节肢动物门;沉积物中主要的无脊椎动物为环节动物门和节肢动物门。沉积物休眠卵OTU丰富度最高,水采样品的OTU丰富度高于网采样品。比较浮游动物的形态鉴定和eDNA鉴定,发现后者在属水平上能鉴定出更多的种类;形态学鉴定的桡足类(100%)和多数轮虫(58.82%)可被eDNA注释出,而eDNA注释出的多数桡足类(71.43%)和轮虫(58.82%)在形态学鉴定中未发现;eDNA未注释出枝角类。结果表明,eDNA在无脊椎动物调查中具有较高的应用潜力,与传统形态学鉴定组合应用能更全面地了解无脊椎动物的种类和水生生物多样性。 |
| 关键词: 广州海珠国家湿地公园 环境DNA 无脊椎动物 浮游动物 生物多样性 |
| DOI：10.18307/2023.0437 |
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| 基金项目:广东省基础与应用基础研究基金项目(2022A1515011387)和南方海洋科学与工程广东省实验室(珠海)创新团队建设项目(311020006)联合资助。 |
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| Biodiversity of aquatic invertebrates based on environmental DNA metabarcoding technology: A case study of Lake Haizhu in Guangzhou |
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Tang Shiqin, Wang Qing, Liu Lu, Yang Yufeng
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Department of Ecology, Key Laboratory of Philosophy and Social Science in Guangdong Province of Jinan University, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangzhou 510632, P. R. China
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| Abstract: |
| The diversity and community structure of invertebrates in the water and sediment of Lake Haizhu in the Guangzhou Haizhu National Wetland Park was investigated based on environmental DNA metabarcoding technology (eDNA), and the detection ability for zooplankton was compared between eDNA and traditional morphological identification. The results showed that a total of 9 phyla, 16 classes, 34 orders, 71 families, 93 genera and 137 operational taxonomic units (OTUs) of aquatic invertebrates were detected by eDNA. In the samples directly collected from water (W), a total of 9 phyla, 50 genera and 68 OTUs were detected; in the samples collected by a planktonic net (N), a total of 6 phyla, 27 genera and 35 OTUs were detected; and in the sedimental samples (S), a total of 9 phyla, 70 genera and 103 OTUs were detected. Rotifera and Arthropoda were the major invertebrates in both W and N; Annelida and Arthropoda were the major invertebrates in S. The OTU number was highest in S, followed by W and N. The copepods (100%) and most of rotifers (58.82%) identified by the morphological method could be annotated by eDNA, while most of the copepods (71.43%) and rotifers (58.82%) annotated by eDNA were not detected by the morphological identification; and cladocerans was not annotated by eDNA. The results suggest that eDNA has high potential for invertebrate surveys, and the combination of eDNA and traditional morphological identification provides a more comprehensive understanding of invertebrates and aquatic biodiversity of the lake ecosystem. |
| Key words: Guangzhou Haizhu National Wetland Park environmental DNA invertebrates zooplankton biodiversity |
